Liquid chromatography–tandem mass spectrometry
LC-MS/MS — steroid panel
Laboratory-developed method
Developed and validated in house by Clinicore. Reviewed and approved by the Medical Director before use in patient testing.
Results from a laboratory-developed method are method-specific and are not directly comparable with another laboratory’s. That is the point of it — where a cleared assay answers the question adequately, Clinicore uses one.
How it works
Compounds are separated chromatographically in time, then each is confirmed by a mass-to-charge transition specific to that molecule. The result is a structural measurement of the target analyte rather than an estimate of how strongly an antibody binds.
What this method does well
- Specificity across structurally similar steroids, including isobaric compounds
- Accuracy at low concentrations where immunoassay cross-reacts and runs biased
- Multiplexes a large panel from a single specimen
- Not subject to biotin interference